This study surveyed both heterosexual and homosexual female detainees to determine whether lesbian inmates, particularly those with a masculinized appearance, are more prone to violent or antisocial behavior, than heterosexual females without masculine features and, if so, whether this relationship is a function of biological conditions or sociological influences. Results indicated that lesbian detainees had more masculine traits, more feelings of hostility and anger, and experienced more physical child abuse than did heterosexual detainees. Although they did not report significantly more violent crimes, they were more often detained for longer periods of time. These findings suggest that lesbian detainees are treated more harshly by the criminal justice system.